pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)
(Plasmid
#239362)
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PurposepPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin
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Depositing Lab
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Depositing OrganizationUniversity of Cambridge
Located in the United Kingdom of Great Britain and Northern Ireland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 239362-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 239362-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPOTv7-hyg
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Backbone manufacturern/a
- Backbone size w/o insert (bp) 6280
- Total vector size (bp) 6991
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Vector typebacterial
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRice Auxin inducible degradation domain
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Alt nameAID
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SpeciesRice
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Insert Size (bp)711
- Promoter n/a
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Tags
/ Fusion Proteins
- 3 x Ty epitope tag (N terminal on backbone)
- 3 x Ty epitope tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GCGATAGCTTAGCTATCAG
- 3′ sequencing primer AGTGCACGTACACAGCGGC
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 239362-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 239362-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPOTv6-hyg-3Ty:OsAID:3Ty (p5230) was a gift from Mark Carrington (Addgene plasmid # 239362 ; http://n2t.net/addgene:239362 ; RRID:Addgene_239362) -
For your References section:
An auxin-inducible degron system for trypanosomes. Alexandra Klein, Alice McDowell, Christian R S Reis, Rodrigo Pontes de Lima, Danielle M N Moura, Janaina de Freitas Nascimento, Osvaldo P de Melo Neto, Mark Carrington. bioRxiv 2025.06.17.660065 10.1101/2025.06.17.660065
Map uploaded by the depositor.
