pHS2056
(Plasmid #239837)

• Purpose: OMEGAOff + omegaRNA scaffold AAV expression
• Depositing Lab: Feng Zhang
• Publication: Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3.

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Backbone

• Vector backbone: pAAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
• Species: M. musculus (mouse)
• Mutation: Insertion of REC domain from NbaCas9-1 + E137K/E409R/I533K in OrufIscB, fusion to human DNMT3A-3L and KRAB; Replacement of A12-A57 with GAAA in omegaRNA scaffold and 17 nt truncation at the 3' end of omegaRNA scaffold
• Promoter: EFS; human U6
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry