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pminiTol2-5xUAS-sfGFP-zP2A-zB19G-zT2A-zTVA (UGBT)
(Plasmid #240274)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 240274 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pminiTol2
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    B19G (codon-optimized for Danio rerio)
  • Species
    Synthetic; Rabies virus

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sfGFP (codon-optimized for Danio rerio)
  • Alt name
    Superfolder GFP
  • Species
    Synthetic

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    TVA (codon-optimized for Danio rerio)

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.7554/eLife.100880.1 for the eLife preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pminiTol2-5xUAS-sfGFP-zP2A-zB19G-zT2A-zTVA (UGBT) was a gift from Xufei Du (Addgene plasmid # 240274 ; http://n2t.net/addgene:240274 ; RRID:Addgene_240274)

  • For your References section:

    An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish. Chen TL, Deng QS, Lin KZ, Zheng XD, Wang X, Zhong YW, Ning XY, Li Y, Xu FQ, Du JL, Du XF. eLife 13:RP100880 10.7554/eLife.100880.2
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