pminiTol2-5xUAS-hsp-sfGFP-zP2A-tetoff-zN2cG-zP2A-zTVA (UGNT-A)
(Plasmid #240272)

• Purpose: Helper plasmid for zebrafish rabies virus based-circuit tracing
• Depositing Lab: Xufei Du
• Publication: Chen et al eLife 13:RP100880

Backbone

• Vector backbone: pminiTol2
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: N2cG (codon-optimized for Danio rerio)
• Species: Synthetic; Rabies virus

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: gtgccccaagtgctgctgttc

Gene/Insert 2

• Gene/Insert name: sfGFP (codon-optimized for Danio rerio)
• Alt name: Superfolder GFP
• Species: Synthetic

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: atgagcaagggagaggagctgt

Gene/Insert 3

• Gene/Insert name: TVA (codon-optimized for Danio rerio)
• Species: Synthetic

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: gctagactgctgcctgctctg

Gene/Insert 4

• Gene/Insert name: tetoff (codon-optimized for Danio rerio)

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: atgtctagactggacaagagcaaagtcata

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.7554/eLife.100880.1 for the eLife preprint.