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pAAV EF1-alpha-FLEX_hM3D(Gq)-HA-WPRE3-SV40pA
(Plasmid #240285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240285 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 5416
  • Total vector size (bp) 7284
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gq-coupled hM3D DREADD
  • Alt name
    hM3D(Gq)
  • Species
    Synthetic
  • Insert Size (bp)
    1866
  • Promoter EF1A
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer Human eukaryotic translation elongation factor 1 α1 promoter
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV EF1-alpha-FLEX_hM3D(Gq)-HA-WPRE3-SV40pA was a gift from Andrew Steele (Addgene plasmid # 240285 ; http://n2t.net/addgene:240285 ; RRID:Addgene_240285)

  • For your References section:

    Targeting the peripheral nervous system in adipose tissue with engineered AAV vectors. Willows JW, Lazor LM, Gunsch G, Mishra G, Villa AP, Shay TF, Steele AD, Townsend KL. Cell Rep Methods. 2026 Jul 31:101542. doi: 10.1016/j.crmeth.2026.101542. 10.1016/j.crmeth.2026.101542 PubMed 42537649
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