pvPE-V3
(Plasmid
#240287)
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PurposeEncodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
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Depositing Lab
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Depositing OrganizationJiangxi Agricultural University
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 240287-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 240287-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepvPE-V3
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Alt nameCas9(H840A)-PERV-C RT(D199N, T305K, W312F, V222A, Y63R, C408R)-NCΔH
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SpeciesSynthetic; Cas9 is from S. pyogenes;PERV-C RT is derived from C subtype porcine endogenous retroviruses found in Chinese Bama miniature pig
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Insert Size (bp)5955
- Promoter CMV
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Tags
/ Fusion Proteins
- SV40 NLS (N terminal on backbone)
- SV40 NLS (C terminal on backbone)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7-forward
- 3′ sequencing primer BGH-reverse
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 240287-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 240287-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pvPE-V3 was a gift from Lusheng Huang (Addgene plasmid # 240287 ; http://n2t.net/addgene:240287 ; RRID:Addgene_240287) -
For your References section:
Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase. Liu W, Duan W, Peng Z, Liao Y, Wang X, Liu R, Jing Q, Jiang H, Fan Y, Ge L, Huang L, Xing Y. Trends Biotechnol. 2025 Aug 30:S0167-7799(25)00314-2. doi: 10.1016/j.tibtech.2025.07.029. 10.1016/j.tibtech.2025.07.029 PubMed 40885667
Map uploaded by the depositor.
