Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24054||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameInsulin Receptor
SpeciesH. sapiens (human)
Insert Size (bp)4600
MutationY1158F, Y1162F, Y1163F
Entrez GeneINSR (a.k.a. CD220, HHF5)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The amino acid assignments for the mutations come from an alternate intron that shifts the location of these sites so the K1030R corresponds to other mutations made by others that are K1018R. The clone of the receptor came from William Rutter (Cell 45, 721-732, 1986) who has the same numbering as the Stanley lab. Other researchers do not have the 12 aa alternate intron, and the relevancy of this intron is not fully understood.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HIR Y1158,1162,1163F was a gift from Frederick Stanley (Addgene plasmid # 24054 ; http://n2t.net/addgene:24054 ; RRID:Addgene_24054)
For your References section:Insulin receptor tyrosine kinase activity and phosphorylation of tyrosines 1162 and 1163 are required for insulin-increased prolactin gene expression. Jacob KK, Whittaker J, Stanley FM. Mol Cell Endocrinol. 2002 Jan 15. 186(1):7-16. 10.1016/S0303-7207(01)00674-8 PubMed 11850117
Map uploaded by the depositor.