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HIR K1030R
(Plasmid #24050)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24050 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Insulin Receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4600
  • Mutation
    K1030R
  • Entrez Gene
    INSR (a.k.a. CD220, HHF5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The amino acid assignments for the mutations come from an alternate intron that shifts the location of these sites so the K1030R corresponds to other mutations made by others that are K1018R. The clone of the receptor came from William Rutter (Cell 45, 721-732, 1986) who has the same numbering as the Stanley lab. Other researchers do not have the 12 aa alternate intron, and the relevancy of this intron is not fully understood.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HIR K1030R was a gift from Frederick Stanley (Addgene plasmid # 24050 ; http://n2t.net/addgene:24050 ; RRID:Addgene_24050)
  • For your References section:

    Insulin receptor tyrosine kinase activity and phosphorylation of tyrosines 1162 and 1163 are required for insulin-increased prolactin gene expression. Jacob KK, Whittaker J, Stanley FM. Mol Cell Endocrinol. 2002 Jan 15. 186(1):7-16. 10.1016/S0303-7207(01)00674-8 PubMed 11850117