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HSD2-FLAG
(Plasmid #24097)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24097 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FCIV1
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HSD2
  • Alt name
    11beta hydroxysteroid dehydrogenase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1250
  • Entrez Gene
    HSD11B2 (a.k.a. AME, AME1, HSD11K, HSD2, SDR9C3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI/NheI (unknown if destroyed)
  • 5′ sequencing primer TRC-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HSD2-flag cDNA sequences was subcloned into FCIV1 lentiviral vector. The venus is not fused to the HSD2 and expression of venus is driven by an IRES sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HSD2-FLAG was a gift from Moses Chao (Addgene plasmid # 24097 ; http://n2t.net/addgene:24097 ; RRID:Addgene_24097)
  • For your References section:

    Activation of Trk neurotrophin receptors by glucocorticoids provides a neuroprotective effect. Jeanneteau F, Garabedian MJ, Chao MV. Proc Natl Acad Sci U S A. 2008 Mar 25. 105(12):4862-7. 10.1073/pnas.0709102105 PubMed 18347336