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pc5139-pEGFP-H3.1-K36M
(Plasmid #241430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241430 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 5123
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Histone H3.1
  • Alt name
    H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    408
  • Mutation
    lysine at position 36 to methionine (K36M)
  • GenBank ID
    AAA63185.1
  • Entrez Gene
    H3C1 (a.k.a. H3/A, H3FA, HIST1H3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer AAGAATTCAATGGCTCGTACGAAGCAAAC
  • 3′ sequencing primer AAGCGGCCGCTGCCCTTTCCCCACGGATG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pc5139-pEGFP-H3.1-K36M was a gift from Cristina Cardoso (Addgene plasmid # 241430 ; http://n2t.net/addgene:241430 ; RRID:Addgene_241430)

  • For your References section:

    Dynamic association of H3K36me3 with pericentromeric heterochromatin regulates its replication time. Pradhan SK, Zhang H, Kolobynina KG, Rapp A, Arroyo M, Cardoso MC. EMBO Rep. 2025 Oct;26(20):4950-4976. doi: 10.1038/s44319-025-00575-6. Epub 2025 Sep 8. 10.1038/s44319-025-00575-6 PubMed 40925959
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