|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24175||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepIRESpuro-modified with gateway cassette by recombination clonin
Backbone manufacturerClontech (Unmodified)
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameMouse Polymerase Beta
SpeciesM. musculus (mouse)
Insert Size (bp)1008
Entrez GenePolb (a.k.a. A430088C08Rik)
- Cloning method Gateway Cloning
- 5′ cloning site x (not destroyed)
- 3′ cloning site x (not destroyed)
- 5′ sequencing primer x
- 3′ sequencing primer x (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Mouse pol beta cDNA was cloned into pENTR-D (invitrogen). The mouse pol beta open reading frame was transferred from pENTRD-mPOL beta to a gateway modified pIRES-Puro plasmid via LR recombination reaction.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIRES.Puro.mBeta was a gift from Robert Sobol (Addgene plasmid # 24175 ; http://n2t.net/addgene:24175 ; RRID:Addgene_24175)
For your References section:Parp1 activation in mouse embryonic fibroblasts promotes Pol beta-dependent cellular hypersensitivity to alkylation damage. Jelezcova E, Trivedi RN, Wang XH, Tang JB, Brown AR, Goellner EM, Schamus S, Fornsaglio JL, Sobol RW. Mutat Res. 2010 Jan 22. ():. 10.1016/j.mrfmmm.2010.01.016 PubMed 20096707