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pEMF054
(Plasmid #242107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242107 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pEMF044
  • Modifications to backbone
    Plac:sacB-2 (sacB-1 codon-optimized for C. necator) inserted into the transposon cargo region
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    Potentially toxic at high copy number; recommend propagation in strain with wild-type (low-copy) variant of pir gene
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    TcR
  • Alt name
    tetA
  • Alt name
    tetracycline efflux MFS transporter
  • Species
    Escherichia coli
  • Insert Size (bp)
    1200
  • GenBank ID
    HAJ8325295.1 HAJ8325295.1

Gene/Insert 2

  • Gene/Insert name
    sacB-1
  • Alt name
    levan sucrase
  • Insert Size (bp)
    1422

Gene/Insert 3

  • Gene/Insert name
    sacB-2
  • Alt name
    levan sucrase
  • Alt name
    sacB codon-optimized for C. necator
  • Insert Size (bp)
    1422

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the annotated map in the Supplementary Documents section.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMF054 was a gift from Christopher Johnson (Addgene plasmid # 242107 ; http://n2t.net/addgene:242107 ; RRID:Addgene_242107)

  • For your References section:

    Cas3-mediated genome reduction: demonstration in Cupriavidus necator H16 improves growth on heterotrophic and autotrophic carbon sources. Fulk EM, Swart RM, Nakamura AK, Quinto LB, Sanchez I Nogue V, Calvey CH, Tharun I, Isaacs FJ, Johnson CW. Nucleic Acids Res. 2026 Jul 17;54(14):gkag753. doi: 10.1093/nar/gkag753. 10.1093/nar/gkag753 PubMed 42549571
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