Cas3-mediated genome reduction: demonstration in Cupriavidus necator H16 improves growth on heterotrophic and autotrophic carbon sources.
Fulk EM, Swart RM, Nakamura AK, Quinto LB, Sanchez I Nogue V, Calvey CH, Tharun I, Isaacs FJ, Johnson CW
Nucleic Acids Res. 2026 Jul 17;54(14):gkag753. doi: 10.1093/nar/gkag753.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242105 | pEMF044 | Non-replicative plasmid for random insertion of a Tn5 transposon with tetA and sacB gene (sacB-1) |
| 242106 | pEMF053 | Non-replicative plasmid for random insertion of a Tn5 transposon with tetA and sacB gene codon-optimized for Cupriavidus necator H16 (sacB-2) |
| 242107 | pEMF054 | Non-replicative plasmid for random insertion of a Tn5 transposon with tetA and 2sacB (double sacB cassette) |
| 242173 | pEMF047 | Replicative plasmid for rhamnose-inducible expression of Cascade-cas3 |
| 242190 | pEMF049 | Replicative plasmid for rhamnose-inducible expression of Cascade-cas3 with crRNA targeting sacB-1 |
| 254852 | pEMF009 | pK18msB-based plasmid for replacement of the phaCAB operon in Cupriavidus necator H16 with the chloramphenicol acetyltransferase gene (cat) |
| 254853 | pEMF012 | pK18msB-based plasmid for replacement of the phaCAB operon in Cupriavidus necator H16 with a tetracycline efflux pump gene (tetA) |