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pEMF012
(Plasmid #254853)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254853 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pK18msB (Addgene plasmid # 177839)
  • Backbone size w/o insert (bp) 3237
  • Total vector size (bp) 6292

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tetracycline efflux pump gene
  • Alt name
    tetA
  • Insert Size (bp)
    1200

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains ≈750 bp homology arms flanking the phaCAB locus in the C. necator H16 genome to replace phaCAB with the tetA tetracycline resistance gene. Please see the annotated map in the Supplementary Documents section.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMF012 was a gift from Christopher Johnson (Addgene plasmid # 254853 ; http://n2t.net/addgene:254853 ; RRID:Addgene_254853)

  • For your References section:

    Cas3-mediated genome reduction: demonstration in Cupriavidus necator H16 improves growth on heterotrophic and autotrophic carbon sources. Fulk EM, Swart RM, Nakamura AK, Quinto LB, Sanchez I Nogue V, Calvey CH, Tharun I, Isaacs FJ, Johnson CW. Nucleic Acids Res. 2026 Jul 17;54(14):gkag753. doi: 10.1093/nar/gkag753. 10.1093/nar/gkag753 PubMed 42549571
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