Skip to main content
Addgene
Open Menu

pSBtet-6xHis-TFIIB-V5-BB
(Plasmid #242714)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242714 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pSBtet-BB backbone, Addgene #60505
  • Backbone manufacturer
    Eric Kowarz
  • Backbone size w/o insert (bp) 7804
  • Total vector size (bp) 7152
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transcription Factor IIB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1011
  • Mutation
    Construct is resistant to Dharmacon Accell and OnTarget GTF2B siRNA pools (proprietary sequences)
  • Entrez Gene
    GTF2B (a.k.a. TF2B, TFIIB)
  • Promoter Tight TRE promoter
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • V5 (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesized by Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use with the sleeping beauty transposition system.

Please visit https://doi.org/10.1101/2024.01.16.575933 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBtet-6xHis-TFIIB-V5-BB was a gift from Britt Glaunsinger (Addgene plasmid # 242714 ; http://n2t.net/addgene:242714 ; RRID:Addgene_242714)

  • For your References section:

    Cleavage of the RNA polymerase II general transcription factor TFIIB tunes transcription during stress. Gulyas L, Lari A, Shah SB, Glaunsinger BA. Genes Dev. 2026 May 13. doi: 10.1101/gad.353187.125. 10.1101/gad.353187.125 PubMed 42128667
Related items: