Cleavage of the RNA polymerase II general transcription factor TFIIB tunes transcription during stress.
Gulyas L, Lari A, Shah SB, Glaunsinger BA
Genes Dev. 2026 May 13. doi: 10.1101/gad.353187.125.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213919 | pSBbi-GP-TFIIB-2xStrep | Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB |
| 213920 | pSBbi-GP-TFIIB(D207A)-2xStrep | Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(D207A) |
| 213921 | pSBbi-GP-TFIIB(S65A)-2xStrep | Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65A) |
| 213922 | pSBbi-GP-TFIIB(S65E)-2xStrep | Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65E) |
| 213923 | pLJM1-blast-Halo | Lentivector for expression of HaloTag protein |
| 213924 | PLJM1-blast-TFIIB-Halo | Lentivector for expression of TFIIB followed by flexible linker and C-terminal HaloTag |
| 242714 | pSBtet-6xHis-TFIIB-V5-BB | Doxycycline-inducible expression of double-tagged 6xHis-TFIIB-V5 (human). Construct is resistant to Dharmacon OnTarget and Accell GTF2B siRNA pools. |