pPB-CreERT2
(Plasmid
#242849)
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PurposePiggyBac vector for mammalian expression of CreERT2
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Depositing Lab
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Depositing OrganizationUniversity of Texas Southwestern Medical Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPB-PGK-destination
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Backbone manufacturerAustin Smith, Addgene plasmid #60436
- Backbone size w/o insert (bp) 9194
- Total vector size (bp) 9528
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Vector typeMammalian Expression, Cre/Lox, Synthetic Biology
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCreERT2
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Alt nameCre-ER(T2)
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Alt nameCre-ERT2
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SpeciesH. sapiens (human), Synthetic; bacteriophage P1
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Insert Size (bp)1977
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GGTTCGGCTTCTGGCGTGTGACC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CreERT2 was a gift from Siyuan Zhang (Addgene plasmid # 242849 ; http://n2t.net/addgene:242849 ; RRID:Addgene_242849) -
For your References section:
Tumor-intrinsic IFNγ signaling and niche adaptation drive early colonization in ovarian cancer metastasis. Aleksandrovic E, Fross SR, Golomb SM, Ma W, Liu X, Zhao Z, Das NM, Reese TC, Borges HM, Tenekeci A, Yu L, Lopez J, Zhao M, Zhong Z, Dean KM, Lea J, Xu L, Stack MS, Zhang S. Nat Commun. 2026 Sep 3;17(1):10497. doi: 10.1038/s41467-026-77222-8. 10.1038/s41467-026-77222-8 PubMed 42834039