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TurboID-CTNNB1
(Plasmid #242999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242999 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCS2+ N-flag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTNNB1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
  • Tags / Fusion Proteins
    • TurboID (N terminal on insert)
    • Flag Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Alice Ting; TurboID was cloned from plasmid 3xHA-TurboID-NLS_pcDNA3 (Addgene #107171).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TurboID-CTNNB1 was a gift from Stefan Koch (Addgene plasmid # 242999 ; http://n2t.net/addgene:242999 ; RRID:Addgene_242999)

  • For your References section:

    Comprehensive proximity proteomics expand the known interactome of the oncoprotein beta-catenin. Moparthi L, Zhong W, Koch S. Sci Rep. 2026 Sep 1;16(1):27374. doi: 10.1038/s41598-026-68008-5. 10.1038/s41598-026-68008-5 PubMed 42680804
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