Iron Insensitive Control Construct 2
(Plasmid
#243007)
-
PurposeIron Insensitive Control Construct under the same expression promoter used in IronFist
-
Depositing Lab
-
Depositing OrganizationMedical University of Graz
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 243007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 243007-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 243007-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneVB241016-1888hha
-
Backbone manufacturerVector Builder
- Total vector size (bp) 4362
-
Vector typeMammalian Expression, Gateway Cloning
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert namemNeonGreen
-
Insert Size (bp)708
Gene/Insert 2
-
Gene/Insert nameT2A
-
Insert Size (bp)63
Gene/Insert 3
-
Gene/Insert namemCherry
-
Insert Size (bp)711
Resource Information
-
A portion of this plasmid was derived from a plasmid made byWe have ordered it from Vector Builder
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 243007-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 243007-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Iron Insensitive Control Construct 2 was a gift from Roland Malli (Addgene plasmid # 243007 ; http://n2t.net/addgene:243007 ; RRID:Addgene_243007) -
For your References section:
Tracking Intracellular Labile Iron with a Genetically Encoded Fluorescent Reporter System Based on Protein Stability. Akyol A, Cimen S, Gottschalk B, Erdogan YC, Lischnig A, Barton Alston A, Digigow R, Fluhmann B, Eroglu E, Graier WF, Malli R. ACS Sens. 2025 Aug 22;10(8):5854-5861. doi: 10.1021/acssensors.5c01165. Epub 2025 Aug 1. 10.1021/acssensors.5c01165 PubMed 40747620
Map uploaded by the depositor.
