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(Plasmid #24353)


Item Catalog # Description Quantity Price (USD)
Plasmid 24353 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    Neuropspora crassa
  • Insert Size (bp)
  • Tag / Fusion Protein
    • dsRed (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site AvrI (not destroyed)
  • 5′ sequencing primer DsRed1-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pBAC-3xPDsRed-GH146-MCS with FseI-AscI-AvrII multi-cloning site has been
previously described (Hong et al., 2009). QF cDNA with 5’ FseI and 3’ Avr restriction sites was 
amplified from pAC-QF by PCR, and cloned into the FseI/AvrII restriction sites of pBAC-GH146 to
yield pBac-GH146-QF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBac-GH146-QF was a gift from Liqun Luo (Addgene plasmid # 24353 ; ; RRID:Addgene_24353)
  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990