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PCLP-2/NT-IgG (104)
(Plasmid #24423)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24423 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Ig/pEG-BOS
  • Backbone size w/o insert (bp) 7300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCLP-2
  • Alt name
    Endoglycan
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    630
  • Mutation
    N-terminal (acidic) domain of PCLP-2 (1-630 bp) fused to Human IgG1 Fc
  • Entrez Gene
    PODXL2 (a.k.a. PODLX2)
  • Tag / Fusion Protein
    • Human IgG1 Fc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI/NheI (destroyed during cloning)
  • 3′ cloning site BamHI/BclI (destroyed during cloning)
  • 5′ sequencing primer EF-1a Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR from PCLP-2 clone G11H9-11b with primers (CS36 and CS38). Insert had NheI and BclI sites compatible with XbaI and BamHI in vector. Can NOT be excised.

The IgG1 tag was not confirmed in sequencing, but the lab has tested it functionally.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCLP-2/NT-IgG (104) was a gift from Steven Rosen (Addgene plasmid # 24423 ; http://n2t.net/addgene:24423 ; RRID:Addgene_24423)
  • For your References section:

    Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins. Sassetti C, Van Zante A, Rosen SD. J Biol Chem. 2000 Mar 24. 275(12):9001-10. 10.1074/jbc.275.12.9001 PubMed 10722749