pCDM8-GlyCAM-1/IgG
(Plasmid #17841)

• Depositing Lab: Steven Rosen
• Publication: Bistrup et al J Cell Biol. 1999 May 17. 145(4):899-910.

Backbone

• Vector backbone: pCDM8
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4356
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: E.coli containing the P3 element
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GlyCAM-1/IgG
• Alt name: GlyCAM-1 Fc
• Alt name: GlyCAM1
• Species: M. musculus (mouse)
• Insert Size (bp): 600
• Entrez Gene: Glycam1 (a.k.a. Sgp50)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pIg vector (which encodes the Fc part of human IgG1)was received by us in 1994 from David L. Simmons. In 1996 he gave us permission to distribute this vector widely. This was cloned into pCDM8 with GlyCAM-1.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry