pCAG_TVA66T_P2A_eGFP_P2A_N2cG
(Plasmid
#244446)
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PurposepCAG driven expression of TVA 66T receptor, eGFP, and N2c glycoprotein
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAG vector
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameTVA receptor 66T
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SpeciesALV-A
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Insert Size (bp)540
- Promoter pCAG
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer CCGGCATGGTGCGGTTGTTG
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)720
- Promoter pCAG
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer CTGGTCGAGCTGGACGGCGACG
- (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameN2cG
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Alt nameN2c glycoprotein
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SpeciesRabies virus
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Insert Size (bp)1575
- Promoter pCAG
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer ggttcctcaggttcttttgtttg
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG_TVA66T_P2A_eGFP_P2A_N2cG was a gift from Tristan Geiller (Addgene plasmid # 244446 ; http://n2t.net/addgene:244446 ; RRID:Addgene_244446)