pDEST/TO/GFP-NELFE
(Plasmid
#244457)
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PurposeDestination vector for FRT insertion and doxycycline induced expression of NELFE cDNA tagged with GFP
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Depositing Lab
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Depositing OrganizationUniversity of Copenhagen
Located in Denmark -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepFRT_DEST_TO_GFP
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Vector typeBacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNELFE
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SpeciesH. sapiens (human)
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Insert Size (bp)1143
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Entrez GeneNELFE (a.k.a. D6S45, NELF-E, RD, RDBP, RDP)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST/TO/GFP-NELFE was a gift from Lea Gregersen (Addgene plasmid # 244457 ; http://n2t.net/addgene:244457 ; RRID:Addgene_244457) -
For your References section:
Oxidative stress triggers RNAPII arrest through PARylation and DNA damage. Thomas QA, Wu L, Lesage E, Iversen HKM, Lopez Martinez D, Kompocholi S, Liu H, Nieto Moreno N, Gregersen LH. Nat Commun. 2026 Aug 10;17(1):9571. doi: 10.1038/s41467-026-76443-1. 10.1038/s41467-026-76443-1 PubMed 42706228