Oxidative stress triggers RNAPII arrest through PARylation and DNA damage.

Thomas QA, Wu L, Lesage E, Iversen HKM, Lopez Martinez D, Kompocholi S, Liu H, Nieto Moreno N, Gregersen LH
Nat Commun. 2026 Aug 10;17(1):9571. doi: 10.1038/s41467-026-76443-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
244455	pDONR-HA1/NELFCD/FKBP/PURO/HA2	Donor plasmid for knock-in insertion of a degron (FKBP for dTAG.v1) tag with a cleavable puromycin resistance gene into the NELFCD human locus.
244456	pDEST/TO/FRT/RPB3-FLAG	Destination vector for FRT insertion and doxycycline induced expression of RPB3 (POLR2C) tagged with 3xFLAG.
244457	pDEST/TO/GFP-NELFE	Destination vector for FRT insertion and doxycycline induced expression of NELFE cDNA tagged with GFP