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Lenti-hU6-xCas12m sgRNA(BsmbI)
(Plasmid #244459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244459 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BFP
  • gRNA/shRNA sequence
    clone sgRNA using BsmB1
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-hU6-xCas12m sgRNA(BsmbI) was a gift from Kailong Li (Addgene plasmid # 244459 ; http://n2t.net/addgene:244459 ; RRID:Addgene_244459)

  • For your References section:

    Structure-guided discovery and engineering of miniature CRISPR–Cas12m for epigenome editing. Yu T, Ji M, Yu D, Guan Z, Zhu R, Jiang Y, Yang Z, Qiu L, Zhang Z, Mu J, Mao F, Xiang K, Bai L, Li K. Nat Struct Mol Biol (2026). 10.1038/s41594-026-01890-9
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