pEGFP-2xFYVE
(Plasmid
#244941)
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PurposeEGFP-FYVE transient transfection as PI3P sensor
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Depositing Lab
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Depositing OrganizationUniversity of Vienna
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name2xFYVE domain (Hrf (mouse) AA147-223)
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Alt nameHGF-regulated tyrosine kinase substrate
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal ID: SMc1390
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-2xFYVE was a gift from Sascha Martens (Addgene plasmid # 244941 ; http://n2t.net/addgene:244941 ; RRID:Addgene_244941) -
For your References section:
A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation. Holzer E, Sawa-Makarska J, Bernklau D, Romanov J, Schuschnig M, Martens S. Nat Commun. 2026 Aug 31;17(1):10374. doi: 10.1038/s41467-026-77368-5. 10.1038/s41467-026-77368-5 PubMed 42816491