A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation.
Holzer E, Sawa-Makarska J, Bernklau D, Romanov J, Schuschnig M, Martens S
Nat Commun. 2026 Aug 31;17(1):10374. doi: 10.1038/s41467-026-77368-5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244939 | pGEX4T3_GST-p62 aa 334-342_LIR region | Protein purification of p62 LIR region |
| 244940 | pFastBac_6xHis-TEV-ATG9A-GFP-StrepII | Generation of Bacmids for the purification of ATG9A-GFP-StrepII from Insect cells |
| 244941 | pEGFP-2xFYVE | EGFP-FYVE transient transfection as PI3P sensor |
| 244942 | pUC19_ATG9A Cterm homology arms_GFP_FLAG | CRISPR knock in of mEGFP-3C-Flag on the C terminus of ATG9A |
| 244943 | pFastBac_6xHis-TEV-WIPI4 | Protein purification of WIPI4 (Insect cells after Bacmid generation) |
| 244944 | pETDuet_6xHis-TEV-mCh-2xFYVE | Protein purification of mCh-2xFYVE domain as sensor for PI3KC3-C1 activity |
| 244945 | pcDNA_3xFLAG-TEV-ATG2A-mCherry | Protein purification and transient transfection of ATG2A-mCh |
| 244946 | pGEX4T1_GST-TEV-mCherry-GABARAP GLY EXPOSED | Protein purification of mCh-GABARAP aa1-116 |
| 244947 | pGEX4T1_GST-TEV-mCherry-GABARAP full-length | Protein purification of mCh-GABARAP fl |
| 244948 | pGEX4T1_GST-TEV-GABARAP full-length | Protein purification of GABARAP fl |
| 244949 | pcDNA_3XFLAG-TEV-ATG2A | Protein purification of ATG2A |
| 244950 | pGEX4T1_GST-Thrombin-LC3B-6xHis | Protein purification of LC3B aa1-120-6xHis |
| 244951 | pcDNA_3XFLAG-TEV-ATG2A_LIRmut | Protein purification of ATG2A LIRmut |
| 244952 | pcDNA_3XFLAG-TEV-ATG2A-LIRmut-mCherry | Protein purification of ATG2A LIRmut-mCherry |