pGEX4T1_GST-TEV-mCherry-GABARAP full-length
(Plasmid
#244947)
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PurposeProtein purification of mCh-GABARAP fl
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Depositing Lab
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Depositing OrganizationUniversity of Vienna
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry-GABARAP
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SpeciesH. sapiens (human)
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Entrez GeneGABARAP (a.k.a. ATG8A, GABARAP-a, MM46)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal ID: SMc2408
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4T1_GST-TEV-mCherry-GABARAP full-length was a gift from Sascha Martens (Addgene plasmid # 244947 ; http://n2t.net/addgene:244947 ; RRID:Addgene_244947) -
For your References section:
A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation. Holzer E, Sawa-Makarska J, Bernklau D, Romanov J, Schuschnig M, Martens S. Nat Commun. 2026 Aug 31;17(1):10374. doi: 10.1038/s41467-026-77368-5. 10.1038/s41467-026-77368-5 PubMed 42816491