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pUC19_ATG9A Cterm homology arms_GFP_FLAG
(Plasmid #244942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244942 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG9A left homology arm - mEGFP-3C-Flag - right homology arm
  • Species
    H. sapiens (human)
  • Entrez Gene
    ATG9A (a.k.a. APG9L1, MGD3208, mATG9)
  • Tag / Fusion Protein
    • GFP-3C-FLAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal ID: SMc1430

Two nucleotide discrepancies were found in the left and right homology arm. They do not affect function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_ATG9A Cterm homology arms_GFP_FLAG was a gift from Sascha Martens (Addgene plasmid # 244942 ; http://n2t.net/addgene:244942 ; RRID:Addgene_244942)

  • For your References section:

    A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation. Holzer E, Sawa-Makarska J, Bernklau D, Romanov J, Schuschnig M, Martens S. Nat Commun. 2026 Aug 31;17(1):10374. doi: 10.1038/s41467-026-77368-5. 10.1038/s41467-026-77368-5 PubMed 42816491
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