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pGEX4T1_GST-Thrombin-LC3B-6xHis
(Plasmid #244950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244950 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LC3B aa1-120-6xHis
  • Alt name
    MAP1LC3B
  • Species
    H. sapiens (human)
  • Mutation
    del AA121-125
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal ID: SMc2649

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1_GST-Thrombin-LC3B-6xHis was a gift from Sascha Martens (Addgene plasmid # 244950 ; http://n2t.net/addgene:244950 ; RRID:Addgene_244950)

  • For your References section:

    A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation. Holzer E, Sawa-Makarska J, Bernklau D, Romanov J, Schuschnig M, Martens S. Nat Commun. 2026 Aug 31;17(1):10374. doi: 10.1038/s41467-026-77368-5. 10.1038/s41467-026-77368-5 PubMed 42816491
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