Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24503||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRASSL Ro2
SpeciesH. sapiens (human)
Mutationmutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
Entrez GeneOPRK1 (a.k.a. K-OR-1, KOR, KOR-1, OPRK)
/ Fusion Proteins
- FLAG (N terminal on insert)
- NLS from prolactin (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI/XhoI (not destroyed)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt
Prolactin NLS sequence: MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS
For more information about this plasmid see: http://openwetware.org/wiki/R02-ORF
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-Ro2 was a gift from Bruce Conklin (Addgene plasmid # 24503 ; http://n2t.net/addgene:24503 ; RRID:Addgene_24503)
For your References section:Controlling signaling with a specifically designed Gi-coupled receptor. Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR.. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7. 10.1073/pnas.95.1.352 PubMed 9419379