pAAVS-iBiT-Lg-MCS-BSD
(Plasmid
#245343)
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Purpose(Empty Backbone) CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBluescript
- Backbone size (bp) 8067
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Vector typeMammalian Expression, Luciferase
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Selectable markersBlasticidin
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Tag
/ Fusion Protein
- Large BiT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMK243 (Tet-OsTIR1-PURO) was a gift from Masato Kanemaki (Addgene plasmid # 72835 ; http://n2t.net/addgene:72835 ; RRID:Addgene_72835)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAVS-iBiT-Lg-MCS-BSD was a gift from Lienhard Schmitz (Addgene plasmid # 245343 ; http://n2t.net/addgene:245343 ; RRID:Addgene_245343) -
For your References section:
iBiT: A vector system allowing stable genome integration and scalable expression of NanoLuc fusion proteins to monitor dynamic protein-protein interactions in intact cells. Dreute J, Schmitz ML. Plasmid. 2026 May 23;135:102772. doi: 10.1016/j.plasmid.2026.102772. 10.1016/j.plasmid.2026.102772 PubMed 42178036