iBiT: A vector system allowing stable genome integration and scalable expression of NanoLuc fusion proteins to monitor dynamic protein-protein interactions in intact cells.
Dreute J, Schmitz ML
Plasmid. 2026 May 23;135:102772. doi: 10.1016/j.plasmid.2026.102772.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245343 | pAAVS-iBiT-Lg-MCS-BSD | CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19 |
| 245344 | pAAVS-iBiT-MCS-Lg-BSD | CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19 |
| 245345 | pAAVS-iBiT-MCS-Sm-Puro | CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19 |
| 245346 | pAAVS-iBiT-Sm-MCS-Puro | CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19 |
| 245347 | pAAVS-iBiT-p62(iso1)-Lg-BSD | (Positive control for protein-protein interaction) CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19 |
| 245348 | pAAVS-iBiT-p62(iso2)-Sm-Puro | (Positive control for protein-protein interaction) CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19 |