wc-2[mAp]_hph
(Plasmid
#245516)
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PurposeEndogenous C-terminal tag the circadian clock positive arm component WC-2 with mApple.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRS426
- Total vector size (bp) 10742
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Vector typeNeurospora Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewc-2
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Alt nameNCU00902
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SpeciesNeurospora crassa
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Insert Size (bp)5100
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GenBank IDXM_958726
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Tag
/ Fusion Protein
- mApple (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gggttttcccagtcacgacgcgaggccattgcgacg
- 3′ sequencing primer aatttcacacaggaaacagcggcgagtctggacgctg
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
wc-2[mAp]_hph was a gift from Jay C Dunlap (Addgene plasmid # 245516 ; http://n2t.net/addgene:245516 ; RRID:Addgene_245516) -
For your References section:
Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia. Wang Z, Bartholomai BM, Wang B, Ritz D, Schultz D, Loros JJ, Dunlap JC. J Cell Biol. 2026 Jun 1;225(6):e202509044. doi: 10.1083/jcb.202509044. Epub 2026 May 19. 10.1083/jcb.202509044 PubMed 42154498