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csr-1_frq[mCherry_V5H6]
(Plasmid #245517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245517 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRS426
  • Total vector size (bp) 10751
  • Vector type
    Neurospora Expression
  • Selectable markers
    Cyclosporin A

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    frq
  • Alt name
    NCU02265
  • Species
    Neurospora crassa
  • Insert Size (bp)
    9087
  • GenBank ID
    XM_011396823
  • Promoter frq promoter
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • V5H6 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gctggagaagctcattccacc
  • 3′ sequencing primer caaccgagcacaccaactaacc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    csr-1_frq[mCherry_V5H6] was a gift from Jay C Dunlap (Addgene plasmid # 245517 ; http://n2t.net/addgene:245517 ; RRID:Addgene_245517)

  • For your References section:

    Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia. Wang Z, Bartholomai BM, Wang B, Ritz D, Schultz D, Loros JJ, Dunlap JC. J Cell Biol. 2026 Jun 1;225(6):e202509044. doi: 10.1083/jcb.202509044. Epub 2026 May 19. 10.1083/jcb.202509044 PubMed 42154498
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