wc-1[mNG_Flag]_bar
(Plasmid
#245518)
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PurposeEndogenous C-terminal tag the circadian clock positive arm component WC-1 with condon-optimized mNeonGreen and Flag tag.
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRS426
- Total vector size (bp) 10742
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Vector typeNeurospora Expression
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Selectable markersphosphinothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewc-1
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Alt nameNCU02356
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SpeciesNeurospora crassa
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Insert Size (bp)7920
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GenBank IDXM_011396849
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Tags
/ Fusion Proteins
- mNeonGreen (C terminal on insert)
- Flag (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
wc-1[mNG_Flag]_bar was a gift from Jay C Dunlap & Bin Wang (Addgene plasmid # 245518 ; http://n2t.net/addgene:245518 ; RRID:Addgene_245518) -
For your References section:
Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia. Wang Z, Bartholomai BM, Wang B, Ritz D, Schultz D, Loros JJ, Dunlap JC. J Cell Biol. 2026 Jun 1;225(6):e202509044. doi: 10.1083/jcb.202509044. Epub 2026 May 19. 10.1083/jcb.202509044 PubMed 42154498