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pHPdestmCherry
(Plasmid #24567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24567 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 24567-D50 50 μg of DNA in Tris buffer $495
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DNA 24567-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGreen H-Pelican
  • Backbone manufacturer
    Scott Barolo
  • Backbone size w/o insert (bp) 10462
  • Vector type
    reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    For all constructs with a Gateway RfA cassette you need DB3.1 bacterial cells for transformation or any other E. coli strain that is resistant to CcdB effects.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    pHPdestmCherry
  • Species
    reporter gene mCherry
  • Insert Size (bp)
    1722
  • GenBank ID
    GU142866
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AmpR in backbone; CmR in gateway RfA cassette

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHPdestmCherry was a gift from Ingrid Lohmann (Addgene plasmid # 24567 ; http://n2t.net/addgene:24567 ; RRID:Addgene_24567)

  • For your References section:

    Vectors for efficient and high-throughput construction of fluorescent Drosophila reporters using the PhiC31 site-specific integration system. Boy AL, Zhai Z, Habring-Müller A, Kussler-Schneider Y, Kaspar P, Lohmann I.. Genesis. 2010 Apr 22. [Epub ahead of print] 10.1002/dvg.20637 PubMed 20506180
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