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Epac-S-H334
(Plasmid #245738)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245738 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Tol2
  • Backbone size w/o insert (bp) 6866
  • Total vector size (bp) 12280
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTurq2del_EPACdDEPCD_Q270E_L777A_K778A_tdblcp173V(st)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5420
  • Mutation
    Deletion of DEP domain, Catalytically dead T781A & F782A, increased affinity for cAMP Q270E, no nuclear envelope affinity L777A & K778A, black Venus Y145W
  • Promoter EF-1a
  • Tag / Fusion Protein
    • T2A, Photoactivatable mCherry-3xNLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site KpnI (destroyed during cloning)
  • 5′ sequencing primer TTCGAAAGCCCCCAGGTCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.11.664338 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Epac-S-H334 was a gift from Kees Jalink (Addgene plasmid # 245738 ; http://n2t.net/addgene:245738 ; RRID:Addgene_245738)

  • For your References section:

    Beyond Static Screens: A High-Throughput Pooled Imaging CRISPR Platform for Dynamic Phenotype Discovery. Mukherjee S, van Tooren M, Zanetti G, Sfakianakis D, Kemps D, Klarenbeek J, Kuiken HJ, Lieftink C, van den Broek B, Beijersbergen RL, Jalink K. bioRxiv 2025.07.11.664338 10.1101/2025.07.11.664338
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