Beyond Static Screens: A High-Throughput Pooled Imaging CRISPR Platform for Dynamic Phenotype Discovery
Mukherjee S, van Tooren M, Zanetti G, Sfakianakis D, Kemps D, Klarenbeek J, Kuiken HJ, Lieftink C, van den Broek B, Beijersbergen RL, Jalink K
bioRxiv 2025.07.11.664338
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245738 | Epac-S-H334 | FLIM biosensor for real-time cAMP monitoring, no nuclear membrane affinity, with nuclear photoactivatable mCherry. mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST)-2A-PAmCherry-3xNLS |
| 245739 | Epac-S-H250 | FLIM biosensor for real-time cAMP monitoring, with nuclear photoactivatable mCherry to label nuclei of selected cells. mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Venus(ST)-2A-PAmCherry-HNS |