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mTalin1(F2F3)_L353F
(Plasmid #245891)

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Item Catalog # Description Quantity Price (USD)
Plasmid 245891 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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Backbone

  • Vector backbone
    pET151 dTOPO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLN1
  • Species
    M. musculus (mouse)
  • Mutation
    L353F
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Promoter T7
  • Tag / Fusion Protein
    • HisTag-V5 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTalin1(F2F3)_L353F was a gift from Ben Goult (Addgene plasmid # 245891 ; http://n2t.net/addgene:245891 ; RRID:Addgene_245891)

  • For your References section:

    De novo talin-1 variant L353F connects multifaceted clinical symptoms to alterations in talin-1 function. Athale M, Ball N, Azizi L, Valenzuela I, Codina M, Martin-Nalda A, Mykuliak VV, Rahikainen R, Goult BT, Turkki P, Hytonen VP. Biochem J. 2025 Sep 17;482(18):1337-1352. doi: 10.1042/BCJ20253128. 10.1042/BCJ20253128 PubMed 40960860
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