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mVD1_I12KA50I_eGFP
(Plasmid #245896)

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Item Catalog # Description Quantity Price (USD)
Plasmid 245896 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VD1
  • Species
    M. musculus (mouse)
  • Mutation
    I12K, A50I
  • Entrez Gene
    Vcl (a.k.a. 9430097D22)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP-Avitag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.05.07.722930 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mVD1_I12KA50I_eGFP was a gift from Ben Goult (Addgene plasmid # 245896 ; http://n2t.net/addgene:245896 ; RRID:Addgene_245896)

  • For your References section:

    Talin controls the spatial distribution of vinculin tension in focal adhesions. Kallem T, Guo Y, Reynolds MK, Ball NJ, Athale M, Baker KB, Mykuliak VV, Ek F, Aldaz-Casanova S, Turkki P, Hytönen VP, Brown NH, Hoffman BD, Yan J, Goult BT. bioRxiv 2026.05.07.722930 10.64898/2026.05.07.722930
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