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VinTS_I12KA50I
(Plasmid #245897)

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Item Catalog # Description Quantity Price (USD)
Plasmid 245897 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VinTS
  • Species
    G. gallus (chicken)
  • Mutation
    I12K, A50I
  • Entrez Gene
    Vcl (a.k.a. 9430097D22)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Site-directed mutagenesis was used to install I12K mutation onto the VinTS-A50I vector (Addgene 111827).

Please visit https://doi.org/10.64898/2026.05.07.722930 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VinTS_I12KA50I was a gift from Ben Goult (Addgene plasmid # 245897 ; http://n2t.net/addgene:245897 ; RRID:Addgene_245897)

  • For your References section:

    Talin controls the spatial distribution of vinculin tension in focal adhesions. Kallem T, Guo Y, Reynolds MK, Ball NJ, Athale M, Baker KB, Mykuliak VV, Ek F, Aldaz-Casanova S, Turkki P, Hytönen VP, Brown NH, Hoffman BD, Yan J, Goult BT. bioRxiv 2026.05.07.722930 10.64898/2026.05.07.722930
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