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Addgene

pLAIΔmls
(Plasmid #24594)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24594 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 7297

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HIV-1
  • Species
    Human immunodeficiency virus 1
  • Mutation
    Human immunodeficiency virus type 1, isolate BRU, complete genome(LAV-1). Large deletion in the pol ORF.

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    NIH AIDS Research and Reference Reagent Program

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A deletion with Mls1(MscI) was made in the provirus vector and the backbone was then religated to produce a replication deficient virus vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLAIΔmls was a gift from Alan Cochrane (Addgene plasmid # 24594 ; http://n2t.net/addgene:24594 ; RRID:Addgene_24594)