pSRG101 (KasI::24xGCN4::T2A::AvrII)
(Plasmid
#246458)
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Purpose24xGCN4::T2A with flanking restriction sites (KasI and AvrII). Useful for cloning SunTag reporter plasmids for expression in C. elegans.
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Depositing Lab
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Depositing OrganizationUtrecht University
Located in Netherlands -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246458 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBSK
- Backbone size w/o insert (bp) 3107
- Total vector size (bp) 4928
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name24xGCN4::T2A
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Alt nameSunTag epitopes
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Insert Size (bp)1821
Resource Information
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A portion of this plasmid was derived from a plasmid made by24xGCN4 is from Addgene plasmid #61058, a kind gift from Tanenbaum.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSRG101 (KasI::24xGCN4::T2A::AvrII) was a gift from Suzan Ruijtenberg (Addgene plasmid # 246458 ; http://n2t.net/addgene:246458 ; RRID:Addgene_246458) -
For your References section:
SunTag-Based Single-Molecule Translation Imaging in Caenorhabditis elegans. van der Salm E, Kontogiannis S, Ruijtenberg S. Bio Protoc. 2025 Oct 20;15(20):e5486. doi: 10.21769/BioProtoc.5486. eCollection 2025 Oct 20. 10.21769/BioProtoc.5486 PubMed 41189716
