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Suzan Ruijtenberg Lab: SunTag-Based Single-Molecule Translation Imaging in Caenorhabditis elegans.

van der Salm E, Kontogiannis S, Ruijtenberg S
Bio Protoc. 2025 Oct 20;15(20):e5486. doi: 10.21769/BioProtoc.5486. eCollection 2025 Oct 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246457pSRG100 (germline optimized 3xGCN4 for C. elegans)Encodes germline optimized 3xGCN4 for C. elegans. Useful to make CRISPR/Cas9 repair templates for 3xGCN4 integration in a gene of interest. For SunTag live-imaging of translation or protein labelling.
246458pSRG101 (KasI::24xGCN4::T2A::AvrII)24xGCN4::T2A with flanking restriction sites (KasI and AvrII). Useful for cloning SunTag reporter plasmids for expression in C. elegans.

Antibodies from Article