pCMV5 Flag-Par6 delta 250-295 Flag tagged
(Plasmid #24646)

• Depositing Lab: Jeff Wrana
• Publication: Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Par6c
• Alt name: Pard6a
• Species: M. musculus (mouse)
• Mutation: Deletion of the PDZ domain of Par6c (aa 250-295)
• Entrez Gene: Pard6a (a.k.a. 0710008C04Rik, 2610010A15Rik, PAR-6A, PAR6alpha, Par-6, Par6, Par6c, TAX40, Tip-40)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PCR was performed using pcDNA3-mPar6c from Tony Pawson's lab as the template. Backbone is from pCMV5B-flag-Smurf1 constructs.