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pKLV2-EF1a-neoT2AFlagStop-W
(Plasmid #246510)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246510 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pKLV2 lentiviral vector
  • Backbone manufacturer
    Kosuke Yusa
  • Backbone size (bp) 8948
  • Modifications to backbone
    Human EF1a promoter-Neo-T2A-Flag followed by the NotI cloning site is inserted.
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter EF-1alpha
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV2-EF1a-neoT2AFlagStop-W was a gift from Kosuke Yusa (Addgene plasmid # 246510 ; http://n2t.net/addgene:246510 ; RRID:Addgene_246510)

  • For your References section:

    CDK10 loss primes tumor cells for transcriptional vulnerability and sensitizes to CDK12/13 inhibition. Islam S, Tarumoto Y, Takano M, Sugino S, Nishibuchi G, Mizutani A, Yamakawa H, Morishita D, Yusa K. Cell Death Dis. 2026 Jun 11;17(1):737. doi: 10.1038/s41419-026-08973-x. 10.1038/s41419-026-08973-x PubMed 42270604
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