pAAV-hSyn-JEDI3sub-WPRE
(Plasmid #246619)

• Purpose: Genetically encoded voltage indicator (GEVI) JEDI3sub in an AAV production vector for neuron -specific expression using the promoter hSyn
• Depositing Lab: Francois St-Pierre
• Publication: Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s41592-026-03043-8.

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Backbone

• Vector backbone: pAAV-hSyn
• Backbone size w/o insert (bp): 4551
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: JEDI3sub
• Species: Synthetic
• Insert Size (bp): 1281
• Promoter: hSyn

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CGCGAGATAGGGGGGCA
• 3′ sequencing primer: caaaggcattaaagcagcgtatc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was tested only in dissociated hippocampal neurons in the corresponding publication.