Designer indicators for two-photon recording of subthreshold voltage dynamics.
Land MA, Galdamez M, Villette V, Zhu J, Lu X, Marosi M, Yang S, Foran G, McDonald AJ, Dong X, Zaabout E, Liu H, Liu Z, Colbert KL, Lai S, Shorey M, Lourdiane ASG, Ayon A, Bradley J, Mailhes-Hamon C, Natan RG, Zhong J, Kroeger R, Law RG, Hakam N, Smith CL, Hu M, Tabb S, Bathellier B, Dudok B, Ji N, Bourdieu L, Reimer J, St-Pierre F
Nat Methods. 2026 Apr 15. doi: 10.1038/s41592-026-03043-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246616 | pAAV-EF1a-DIO-JEDI3sub-Kv-WPRE | Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector expressed under the mammalian promoter (EF1a) |
| 246617 | pAAV-EF1a-DIO-JEDI3sub-WPRE | Double floxed genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector under the control of the mammalian promoter (EF1a) |
| 246618 | pAAV-hSyn-JEDI3sub-Kv-WPRE | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector for neuron -specific expression using the promoter hSyn |
| 246619 | pAAV-hSyn-JEDI3sub-WPRE | Genetically encoded voltage indicator (GEVI) JEDI3sub in an AAV production vector for neuron -specific expression using the promoter hSyn |
| 246620 | pAAV-Ef1a-fDIO-JEDI3sub-WPRE | Flp dependent genetically encoded voltage indicator (GEVI) JEDI3sub in an AAV production vector under the control of the mammalian promoter (EF1a) |
| 246621 | pTol2-HuC(elavl3)-JEDI3sub | Genetically encoded voltage indicator (GEVI) JEDI3sub under the HuC(elavl3) promoter for pan neuronal expression in zebrafish |
| 246622 | pTol2-HuC(elavl3)-JEDI3sub-Kv | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub under the HuC(elavl3) promoter for pan neuronal expression in zebrafish |
| 246623 | pJFRC7-JEDI3sub | Genetically encoded voltage indicator (GEVI) JEDI3sub under the promoter hsp70 for Drosophila (insect) expression |
| 246624 | pAAV-CaMKIIa-JEDI3sub-WPRE | Genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa |
| 246625 | pAAV-CaMKIIa-JEDI3sub-Kv-WPRE | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa |
| 246626 | pcDNA3.1/Puro-CAG-JEDI3sub-CyOFP1 | Genetically encoded voltage indicator (GEVI) JEDI3sub with a 3xGSS linked CyOFP1 expressed under a strong mammalian promoter (CAG) |
| 246627 | pcDNA3.1/Puro-CAG-JEDI3sub | Genetically encoded voltage indicator (GEVI) JEDI3sub expressed under strong mammalian promoter (CAG) |
| 246628 | pcDNA3.1/Puro-CAG-JEDI3sub-mBeRFP(E6F) | Genetically encoded voltage indicator (GEVI) JEDI3sub with a 3xGSS linked mBeRFP E6F expressed under a strong mammalian promoter (CAG) |
| 246629 | pAAV-EF1a-DIO-JEDI3hyp-Kv-WPRE | Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector expressed under the mammalian promoter (EF1a) |
| 246630 | pAAV-EF1a-DIO-JEDI3hyp-WPRE | Double floxed genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector under the control of the mammalian promoter (EF1a) |
| 246631 | pAAV-hSyn-JEDI3hyp-Kv-WPRE | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector for neuron -specific expression using the promoter hSyn |
| 246632 | pAAV-hSyn-JEDI3hyp-WPRE | Genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector for neuron -specific expression using the promoter hSyn |
| 246633 | pAAV-Ef1a-fDIO-JEDI3hyp-WPRE | Flp dependent genetically encoded voltage indicator (GEVI) JEDI3hyp in an AAV production vector under the control of the mammalian promoter (EF1a) |
| 246634 | pTol2-HuC(elavl3)-JEDI3hyp | Genetically encoded voltage indicator (GEVI) JEDI3hyp under the HuC(elavl3) promoter for pan neuronal expression in zebrafish |
| 246635 | pTol2-HuC(elavl3)-JEDI3hyp-Kv | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3hyp under the HuC(elavl3) promoter for pan neuronal expression in zebrafish |
| 246636 | pJFRC7-JEDI3hyp | Genetically encoded voltage indicator (GEVI) JEDI3hyp under the promoter hsp70 for Drosophila (insect) expression |
| 246637 | pAAV-CaMKIIa-JEDI3hyp-WPRE | Genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa |
| 246638 | pAAV-CaMKIIa-JEDI3hyp-Kv-WPRE | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa |
| 246639 | pcDNA3.1/Puro-CAG-JEDI3hyp-CyOFP1 | Genetically encoded voltage indicator (GEVI) JEDI3hyp with a 3xGSS linked CyOFP1 expressed under a strong mammalian promoter (CAG) |
| 246640 | pcDNA3.1/Puro-CAG-JEDI3hyp | Genetically encoded voltage indicator (GEVI) JEDI3hyp expressed under strong mammalian promoter (CAG) |
| 246641 | pcDNA3.1/Puro-CAG-JEDI3hyp-mBeRFP(E6F) | Genetically encoded voltage indicator (GEVI) JEDI3hyp with a 3xGSS linked mBeRFP E6F expressed under a strong mammalian promoter (CAG) |